Original scientific paper

https://doi.org/10.2478/aiht-2022-73-3650

Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects

Jelena Radovanović orcid.org/0000-0002-3005-7943 ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia 2 University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
Biljana Antonijević ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Katarina Baralić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Marijana Ćurčić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Danijela Đukić-Ćosić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Zorica Bulat ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Dragana Javorac orcid.org/0000-0003-2744-2565 ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Aleksandra Buha Đorđević ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Jelena Kotur-Stevuljević orcid.org/0000-0002-6980-3069 ; University of Belgrade Faculty of Pharmacy, Department of Biochemistry, Belgrade, Serbia
Emina Sudar-Milovanović orcid.org/0000-0002-2273-5310 ; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
Evica Antonijević Miljaković ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Miloš Beloica ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia
Zoran Zoran orcid.org/0000-0003-2622-6043 ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia

Abstract

This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2.-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se givenwith NaF improved MDA, SOD1, and O2.- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity.

Keywords

Cu; Fe; MDA; NaF; O2 .-; oxidative stress; Se; SOD1; sodium fluoride; subacute toxicity; Zn

Hrčak ID:

283982

URI

https://hrcak.srce.hr/283982

Publication date:

29.9.2022.

Article data in other languages: croatian

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