Original scientific paper

https://doi.org/10.5562/cca3911

Determination of naphthoquinones in invasive alien plants Impatiens glandulifera Royle and I. balfourii Hook.f. from Croatia

Željan Maleš ; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Ana-Marija Domijan ; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Ivan Duka ; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Tihana Marić ; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Mirza Bojić ; Department of Medicinal Chemistry, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Hrvoje Rimac ; Department of Medicinal Chemistry, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Božena Mitić ; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9A, Zagreb, Croatia
Dario Hruševar ; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9A, Zagreb, Croatia
Karmela Barišić ; Department of Biochemistry and Haematology, University of Zagreb Faculty of Pharmacy and Biochemistry, Domagojeva 2, Zagreb, Croatia
Donatella Verbanac ; Department of Biochemistry and Haematology, University of Zagreb Faculty of Pharmacy and Biochemistry, Domagojeva 2, Zagreb, Croatia

Abstract

The study aimed to develop an HPLC method for a simultaneous determination of 2-hydroxy-1,4-naphthoquinone (2-HNQ) and 2-methoxy-1,4-naphthoquinone (2-MNQ) in plant material. The method was implemented to determine naphthoquinones (NQs) in leaves and flowers of the invasive alien species Impatiens glandulifera and I. balfourii collected in Croatia. Two NQs extraction methods from plant material were compared: decoction and ultrasonic extraction. Validation parameters indicate that the developed method is reliable for the simultaneous determination of 2-HNQ and 2-MNQ in plant material. The decoction extraction obtained a higher NQs yield than ultrasonic extraction. In the leaves and flowers of I. glandulifera and I. balfourii only 2-MNQ was detected. Both, I. glandulifera and I. balfourii, had higher 2-MNQ concentrations in flowers than in leaves. A significantly higher 2-MNQ concentration was found in I. glandulifera compared to I. balfourii. Therefore, I. glandulifera can be considered a rich source of 2-MNQ whose antitumor potential is established.

Keywords

invasive alien species; 2-hydroxy-1,4-naphthoquinone; 2-methoxy-1,4-naphthoquinone; novel therapeutics; HPLC

Hrčak ID:

286205

URI

https://hrcak.srce.hr/286205

Publication date:

23.11.2022.

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