Veterinary Archives, Vol. 78 No. 4, 2008.
Original scientific paper
Effect of the lethal activity of 32p upon alpha amylase activity and glucose concentration in chicken blood plasma
Marinko Vilić
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Petar Kraljević
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Miljenko Šimpraga
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Abstract
An attempt was made to evaluate whether the activity of alpha-amylase and the concentration of glucose changes in blood plasma of chickens after 32P administration, and whether it helps in the diagnosis of organic or functional liver damage in chicken caused by ionizing radiation before the appearance of clinical simptoms of radiation sickness. Fifty day old hybrid chickens of heavy Jata breeds of both sexes were treated with 32P, administered intramuscularly in a single amount of 333 MBq per kilogram of body mass. Blood samples were taken from the wing vein on days 1, 3, 5, 7 and 10 after administration of 32P. The activity of alpha amylase was determined spectrophotometrically by using the kits produced by «Herbos d.d Sisak» (Croatia). Concentration of glucose was determined photometrically with o-toluidinom, by also using the kits produced by «Herbos d.d. Sisak». Alpha amylase activity in the blood plasma od experimental chickens was decreased from the 3rd
day after administartion of 32P to the 10th day of the experiment. A statistically significant difference was only recorded on the 7th day of the experiment. Concentration of glucose in the blood plasma of 32P treated chickens was signifacantlly increased on the 5th and 7th days of the experiment. The results obtained indicate that alpha amylase activity and concentration of glucose are changed in the blood plasma of 32P treated chickens. It seems that the concentration of glucose alone may serve as indicator of functional and/or morphological liver damage in chickens caused by ionizing radiation before the appearance of clinical symptoms of radiation sickness.
Keywords
phosphorus-32; alpha-amylase; glucose; blood plasma; chickens
Hrčak ID:
26536
URI
Publication date:
20.8.2008.
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