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Rad Croatian Academy of Sciences and Arts. Medical sciences : Medical Sciences, Vol. 559 No. 64-65, 2023.

Original scientific paper

https://doi.org/10.21857/94kl4clz3m

Tapping the potential of bone regeneration by delivery of concentrated extracellular vesicles to the fracture site: A new horizon in bone regeneration therapy research?

Lovorka Grgurević orcid id orcid.org/0000-0002-9814-3178 ; University of Zagreb School of Medicine *
Rudjer Novak orcid id orcid.org/0000-0002-5920-4169 ; Center for Translational and Clinical Research, Department of Proteomics, University of Zagreb School of Medicine
Stela Hrkač ; Department of Clinical Immunology, Allergology and Rheumatology, University Hospital Dubrava
Grgur Salai ; Department of Pulmonology, University Hospital Dubrava
Jadranka Bubić ; BIMIS – Biomedical Research Center Šalata, University of Zagreb School of Medicine
Dinko Mitrečić orcid id orcid.org/0000-0003-4836-1721 ; BIMIS – Biomedical Research Center Šalata, University of Zagreb School of Medicine
Marija Lipar ; Department of Orthopaedic surgery and Ophthalmology, Faculty of Veterinary Medicine University of Zagreb
Tomislav Vlahović ; Clinic of Traumatology, University Hospital Center ”Sestre Milosrdnice”

* Corresponding author.

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Abstract

Background A small pilot study was conducted in order to investigate in situ regenerative potential of extracellular vesicles (EVs) in a rat subcutaneous implant model.
Material and methods An implant device was implanted in the axillary regions of four animals and consisted of a rat ABC enriched with an EV isolate obtained from human patients 7-21 days post bone fracture, or healthy individuals.
Results Upon histological examination of the implanted devices, there were clear evidence of early signs of immature cartilage and bone formation in the implants of fracture-EV treated animals, 21 days post implantation.
Conclusion These preliminary results suggest a potential for near-term innovation in the development of an affordable, non-invasive and completely autologous therapeutic device that can potentially
enhance the existing regenerative capacity of skeletal tissues. EV can be used to concentrate the body’s inherent regenerative potential and utilise it as a personalised self-healing therapy.

Keywords

extracellular vesicles; regeneration; subcutaneous assay; bone; fracture

Hrčak ID:

312649

URI

https://hrcak.srce.hr/312649

Publication date:

22.12.2023.

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