Veterinary Archives, Vol. 94 No. 5, 2024.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.2500
First molecular detection and characterization of fowl adenovirus in commercial broilers in Serbia
Ljiljana Spalević
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
Vesna Milićević
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
Branislav Kureljušić
Dimitrije Glišić
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
Dragica Vojinović
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
Ljubiša Veljović
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
Jelena Maletić
; Scientific Institute of Veterinary Medicine of Serbia, Belgrade, Srbia
*
* Corresponding author.
Abstract
The present study was conducted to reveal and identify the epidemiological features of the fowl adenovirus species circulating in the Belgrade city area. The research included clinical examination, necropsy, pathohistology, enzymelinked immunosorbent assay (ELISA), and PCR analysis. Clinically, general weakness, increased conversion, and poor growth were observed. Postmortem examinations showed multiple necrotic foci in the liver, and swollen and pale kidneys. Pathohistological examination of the liver revealed hepatocellular necrosis, vacuolar degeneration of hepatocytes, cholestasis, and hyperemia. Serological analysis of 210 blood sera confirmed seroconversion in 156 (74.28%) samples against FAdVs, and depending on the farm, it ranged from 23.33 to 100%. Using the PCR method, 105 cloacal swabs were analyzed to determine the virus genome found in 31 (29.52%) samples. Hexon gene sequence analysis identified two species: fowl aviadenovirus D (serotype FAdV-2, FAdV-11) and fowl aviadenovirus E (serotype FadV-8b). Those species are closely related to strains circulating in Hungary, Turkey, and other countries. Our findings suggest that FAdV strains are significantly prevalent and could be an emerging pathogen in Serbia.
Keywords
broilers; fowl adenovirus; epidemiology; Serbia
Hrčak ID:
320611
URI
Publication date:
1.9.2024.
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