Original scientific paper
https://doi.org/10.2478/cjf-2024-0018
APPLICATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR Edwardsiella ictaluri DETECTION IN INDONESIA ENTERIC SEPTICEMIA OF CATFISH
Hidayati Sari Utami
; Fish Quarantine Standards Test Center, Fisheries Quality and Safety Control, Indonesia
*
Rahman Rizky Amalia
; Fish Quarantine Standards Test Center, Fisheries Quality and Safety Control, Indonesia
Nainggolan Ronald
; Fish Quarantine Standards Test Center, Fisheries Quality and Safety Control, Indonesia
Ardiani Trisniaty
; Fish Quarantine Standards Test Center, Fisheries Quality and Safety Control, Indonesia
Asri Tina Yunia
; Fish Quarantine Standards Test Center, Fisheries Quality and Safety Control, Indonesia
Raden Wasito
; Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Indonesia
* Corresponding author.
Abstract
Loop-mediated isothermal amplification (LAMP) method is useful for rapidly detecting Edwardsiella ictalurid infection, especially enteric septicemia of catfish (ESC). This study aims to investigate the LAMP method for E. ictaluri detection in ESC. This research was an experimental study using a total of 55 catfish, consisting of 5 negative controls and 50 catfish injected intraperitoneally with 0.1 mL of E. ictaluri at a concentration of 105 CFU/mL. The kidneys of three fishes were randomly selected 6, 12, 18, 24, 30, 36, 42, 48, and 54 hours after infection with E. ictaluri. In addition, samples were also collected on days 3, 5, and 7. Bacterial analysis was determined by conventional biochemistry (genus and species test), whole-genome sequencing of catfish, and LAMP amplification. All types of data obtained in this study were analyzed by descriptive statistics to compare infected and healthy catfish. The catfish were infected with E. ictaluri after 12 hours of infection according to the LAMP amplification procedure which revealed hemorrhages throughout the body, fins, protruding eyes, necrosis, inflammation of the spleen, liver, intestine, pale gills, gut, abdomen swelling, and tissue necrosis in the upper part of the head. It can be concluded that the LAMP method is more effective than the PCR method for detecting infection with E. ictaluri in catfish.
Keywords
Bacteria; catfish; diagnostic method; fish diseases; molecular diagnosis
Hrčak ID:
323739
URI
Publication date:
16.12.2024.
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