Original scientific paper

https://doi.org/10.24099/vet.arhiv.2447

Development of a new ten amino acid antimicrobial peptide (AMP) against extensively drug-resistant bacteria, Pseudomonas aeruginosa and Acinetobacter baumannii

Ali Hussein Mer ; Department of Nursing, Mergasour Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq
Yousef Mirzaei ; Department of Medical Biochemical Analysis, Cihan University-Erbil, Kurdistan Region, Iraq
Bahia Fattah Maran ; Department of business administration, Soran Technical College, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq
Mohammad Jafari ; Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
Zahra Amirkhani ; Student Research Committee, Larestan University of Medical Sciences, Larestan, Iran
Elaheh Rezaei ; Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
Mohammad-Rafi Bazrafshan ; Department of Nursing, School of Nursing, Larestan University of Medical Sciences, Larestan, Iran
Mehdi Mohsenzadeh ; Gerash Al-Zahra Fertility Center, Gerash University of Medical Sciences, Gerash, Iran
Hajar Haghshenas ; Gerash University of Medical Sciences, Gerash, Iran
Seyed Adnan Kashfi ; Gerash University of Medical Sciences, Gerash, Iran
Aliyar Pirouzi ; Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran *

* Corresponding author.

Abstract

Growing microbial resistance to conventional antimicrobial agents reveals the need for development of novel and alternative therapeutic strategies. In the present study, we developed a new ten amino acid antimicrobial peptide (AMP) and evaluated its antibacterial activity against extensively drug-resistant bacteria, Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii). After synthesis of the AMP, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested using different concentrations (2 to 128 ng/mL) of the AMP. Afterwards, the cell viability and toxicity of the AMP were investigated on primary fibroblast cells using MTT and trypan blue exclusion assays with different concentrations (2 to 128 ng/mL) of the AMP, respectively. The AMP showed MICs≥0.023 and 0.046 ng/mL for P. aeruginosa and MICs≥0.18 ng/mL for A. baumannii. In addition, the AMP exhibited MBCs≥0.05 and 0.09 ng/mL for P. aeruginosa and MBCs≥0.04 and 0.045 ng/mL for A. baumannii. Results from the disk diffusion assay showed 12 and 16 mm for P. aeruginosa and 9 mm for A. baumannii. In vitro cell cytotoxicity and hemolytic activity assays confirmed the safety profile of the AMP (no toxicity or hemolytic activities at concentrations ≤8 ng/mL). Our results suggested that the AMP developed in this study is a reliable and safe antimicrobial agent for the treatment of extensively drug-resistant bacteria, P. aeruginosa and A. baumannii.

Keywords

antibacterial peptide (AMP); antibiotic resistance; cytotoxicity; Pseudomonas aeruginosa (P. aeruginosa); Acinetobacter baumannii (A. baumannii)

Hrčak ID:

326151

URI

https://hrcak.srce.hr/326151

Publication date:

1.1.2025.

Article data in other languages: croatian

Visits: 0 *