Original scientific paper
https://doi.org/10.3325/cmj.2023.64.413
Benign and malignant prostate neoplasms show different spatial organization of collagen
Taras Zadvornyi
orcid.org/0000-0003-3033-3976
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
*
Nataliia Lukianova
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Oleksandr Mushii
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Anna Pavlova
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Olena Voronina
orcid.org/0000-0002-7065-374X
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Vasyl Chekhun
; Department of Monitoring Tumor Process and Therapy Design, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
* Corresponding author.
Abstract
Aim To compare the indicators of the spatial organization
of collagen and its regulating factors between benign and
malignant prostate neoplasms.
Methods The study involved tumor tissue samples from
40 patients with stage II-III prostate cancer (PCa) and 20 patients with benign prostatic hyperplasia (BPH). The localization of collagen was determined with a Masson trichrome
stain. To establish quantitative indicators of the spatial organization of collagen, morphometric studies were carried
out with the CurveAlign and ImageJ programs.
Results PCa tissue had two times lower collagen density
(P<0.0001) and 1.3 times lower levels of collagen alignment (P=0.018) compared with BPH tissue. In PCa tissue,
collagen fibers were shorter (by 24.2%; P<0.001) and thicker (by 15.5%; P<0.001). PCa tissue samples showed significantly higher levels of metalloproteinase (MMP)-2 (by 2.4
times; P=0.001), MMP-8 (by 2.3 times; P=0.007), and MMP13 (by 1.9 times; P=0.004).
Conclusions Collagen matrix spatial organization features, as well as its regulatory factors, could be potential
biomarkers of malignant prostate neoplasms
Keywords
Hrčak ID:
331888
URI
Publication date:
21.12.2023.
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