Original scientific paper

Structure of the T₆ Human Nickel Insulin Derivative at 1.35 Å Resolution

Biserka Prugovečki ; Laboratory of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia
Eleanor J. Dodson ; York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5YW England
Guy G. Dodson ; York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5YW England
Dubravka Matković-Čalogović ; Laboratory of General and Inorganic Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia

Abstract

This paper presents results of the structural investigation on the human insulin hexamer derivative stabilised by nickel coordination. Single crystals of the Ni-insulin derivative were prepared by the hanging drop vapour diffusion crystallisation method using metal-free insulin and nickel(II) acetate tetrahydrate. The low-temperature crystal structure was determined by the single crystal X-ray crystallographic method with data extending to 1.35 Å spacing. The investigated insulin derivative exhibits the T₆ form of insulin and crystallizes in the trigonal system in space group R3, with the unit cell parameters a = b = 81.41 Å and c = 33.75 Å. There are two nickel atoms per insulin hexamer and both are octahedrally coordinated by three Nε2 atoms of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules.

Keywords

insulin derivative; nickel; X-ray structure

Hrčak ID:

39660

URI

https://hrcak.srce.hr/39660

Publication date:

15.7.2009.

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