Original scientific paper
https://doi.org/10.2478/10004-1254-60-2009-1934
Comet Assay in Evaluating DNA Damage Associated With Ischaemia-Reperfusion Injury in Patients Undergoing Coronary Surgery
Bensu Karahalil
orcid.org/0000-0003-1625-6337
; Gazi University, Faculty of Pharmacy, Toxicology Department, Ankara, Turkey
Tulin Gumus
; Ataturk Training and Research Hospital, Ministry of Health, Ankara, Turkey
Esra Emerce
; Gazi University, Faculty of Pharmacy, Toxicology Department, Ankara, Turkey
Seval Izdes
; Ataturk Training and Research Hospital, Ministry of Health, Ankara, Turkey
Orhan Kanbak
; Ataturk Training and Research Hospital, Ministry of Health, Ankara, Turkey
Elvin Kesimci
; Ataturk Training and Research Hospital, Ministry of Health, Ankara, Turkey
Abstract
Ischaemia-reperfusion (I/R) injury is responsible for a number of conditions such as coronary bypass and myocardial infarction, and deaths. Oxygen-free radicals formed during I/R have been proposed as the leading causes of tissue injury, and they play an important role in I/R injury. I/R induces oxidative DNA damage (such as purinic and pyrimidinic base lesions). Comet assay is a suitable and sensitive method for early detection of low-level DNA damage. We used modified alkaline comet assay in peripheral blood lymphocytes and evaluated I/R-induced DNA damage in patients undergoing coronary artery bypass graft (CABG) operation (in vivo model for I/R). No statistically significant difference in DNA damage levels was found before surgery, after anaesthesia, ischemia, reperfusion, and surgery. However, blood lactate levels (assessed in parallel with the comet assay) increased after I/R and did not return to the baseline level. Our findings showed that I/R injury did not induce DNA damage, but increased the lactate levels. This finding suggests that there might be reversible and uncommon necrosis that did not refl ect on overall DNA base damage. Further studies are needed using this approach.
Keywords
coronary artery bypass graft surgery; DNA lesions; I/R; lactate; reactive oxygen species
Hrčak ID:
40678
URI
Publication date:
15.9.2009.
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