Original scientific paper

Adsorption of Vancomycin on Amberlite XAD-16 in a Packed Bed Column

B. Borin ; Lek Pharmaceuticals d.d., a Sandoz Company, Kolodvorska 27, SI-1234 Mengeš, Slovenia
A. Pavko ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, SI-1000 Ljubljana, Slovenia

Abstract

Vancomycin is a glycopeptide used in the prophylaxis and treatment of bacterial infections. It is obtained with fermentation of bacteria called Amycolatopsis orientalis. After fermentation, it is purified of coloured additions and inorganic salts by adsorption to a polymeric adsorbent Amberlite XAD-16. In this work, equilibrium adsorption studies to determine the adsorption capacity were done first. The experimental data fitted best with Langmuir isotherm. Maximum capacity was q0 = 53.76 kg m–3 resin at pH 7. Next, adsorption
was studied in a laboratory packed bed adsorption column. A simple mathematical model taking into account axial dispersion was applied to describe the dynamics of the process with breakthrough curves. Experimental and predicted results were compared, and some parametric sensitivity analysis was made to better understand the process for the purpose of scale up.

Keywords

Adsorption; packed bed column; vancomycin

Hrčak ID:

45410

URI

https://hrcak.srce.hr/45410

Publication date:

23.12.2009.

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