Original scientific paper

https://doi.org/10.2478/v10007-010-0032-9

Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

PAVAGADA JAGANNATHAMURTHY RAMESH ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006
KANAKAPURA BASAVAIAH ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006
NAGARAJU RAJENDRAPRASAD ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006

Abstract

A spectrophotometric method for the determination of doxycycline (DOX) is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate and/or molybdate in Folin-Ciocalteu (F-C) reagent by DOX in alkaline medium. The colored species has an absorption maximum at 770 nm and the system obeys Beer’s law over the concentration range 0.7512.0 μg mL1 DOX. The apparent molar absorptivity is 2.78 × 104 L mol1 cm1. The limit of quantification and detection values are reported to be 0.20 and 0.08 µg mL1, respectively. Over the linear range applicable, the accuracy and precision of the method were evaluated on intra-day and inter-day basis. The reported mean accuracy value was 101.0 ± 1.7 %, the relative error was ≤ 2.7 % and the relative standard deviation was ≤ 2.5 %. Application of the proposed method to bulk powder and commercial pharmaceutical tablets is also presented. No significant difference was obtained between the results of the proposed method and the official BP method. The procedure described in this paper is simple, rapid, accurate and precise.

Keywords

doxycycline hyclate; Folin-Ciocalteu reagent; spectrophotometry

Hrčak ID:

59304

URI

https://hrcak.srce.hr/59304

Publication date:

1.12.2010.

Article data in other languages: croatian

Visits: 3.666 *