Original scientific paper

Hematological and biochemical reference intervals in Dalmatian pramenka sheep estimated from reduced sample size by bootstrap resampling.

Aleksandar Vojta ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Shek-Vugrovečki ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Lada Radin ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Maša Efendić ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Jadranka Pejaković ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Miljenko Šimpraga ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Monitoring of animal health status in organic farming relies in part on the availability of exact reference intervals for key hematological and biochemical blood parameters. Once determined, these intervals provide a baseline for interpreting routine laboratory blood tests in order to assess nutritional status, stress or disease. In this study, reference intervals for hematological and blood biochemical parameters were determined for Dalmatian pramenka, an indigenous Croatian sheep breed. A recently developed nonparametric method based on bootstrap resampling was used for calculation of reference intervals, and its performance was compared with that of a conventional nonparametric method. Hematological and blood biochemical profi les from 114 organically reared Dalmatian pramenka sheep were constructed and used for calculation of reference intervals using the new approach. The new method enabled convenient and reliable determination of reference intervals from a relatively small sample size, without the need for transformation or fi ltering of the raw data. Separate reference ranges for male and female sheep were found to be unnecessary for most of the investigated parameters.

Keywords

reference interval; bootstrap; biochemistry; hematology; sheep; pramenka

Hrčak ID:

66466

URI

https://hrcak.srce.hr/66466

Publication date:

21.2.2011.

Article data in other languages: croatian

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