Izvorni znanstveni članak
https://doi.org/10.15644/asc57/3/2
Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials in vivo
Milena Trutina Gavran
orcid.org/0000-0001-5235-5215
; Zavod za morfologiju i antropologiju Studija dentalne medicine Medicinskog fakulteta Sveučilišta u Mostaru, Bosna i Hercegovina
*
Davor Željezić
; Odjel za mutagenezu Instituta za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Lara Vranić
; Stomatološki fakultet Sveučilišta u Zagrebu, Hrvatska
Dubravka Negovetić Vranić
; Zavod za dječju stomatologiju Stomatološkog fakulteta Sveučilišta u Zagrebu, Hrvatska
Lana Grabarević
; Privatna stomatološka poliklinika, Zagreb, Hrvatska
Danijela Jurić-Kaćunarić
orcid.org/0000-0003-2929-0905
; Privatna stomatološka klinika, Gaggenau, Njemačka
Antonija Tadin
orcid.org/0000-0002-5365-9816
; Zavod za restaurativnu dentalnu medicinu i endodonciju Studija dentalne medicine Medicinskog fakulteta Sveučilišta u Splitu, Hrvatska
Sanja Šegović
; Zavod za endodonciju i restaurativnu dentalnu medicinu Stomatološkog fakulteta Sveučilišta u Zagrebu, Hrvatska
Nada Galić
; Zavod za endodonciju i restaurativnu dentalnu medicinu Stomatološkog fakulteta Sveučilišta u Zagrebu, Hrvatska
* Dopisni autor.
Sažetak
Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months.
Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings.
Ključne riječi
Materials Testing; Composite Resins; Dental Amalgam; Micronucleus Tests; Cytotoxins; cytotoxicity; genotoxicity; composite materials; buccal epithelial cells
Hrčak ID:
308159
URI
Datum izdavanja:
27.9.2023.
Posjeta: 996 *