A comparative study of antimicrobial activity of Melaleuca alternifolia, Achillea millefolium and Cinnnamomum camphora vaginal suppositories

Authors

  • Merima Ibišević Department of Pharmaceutical technology, University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Saša Pilipović The Agency for medicinal products and medical devices, Control laboratory of the Agency in Sarajevo, Bosnia and Herzegovina; Department of Pharmaceutical technology, University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Darja Husejnagić Department of Biology, University of Tuzla, Faculty of Natural Sciences and Mathematics, Tuzla, Bosnia and Herzegovina
  • Fadila Malohodžić University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Alma Kulanić University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Lejla Mustafić University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Ermina Cilović Kozarević Department of Pharmacognosy, University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina
  • Emir Horozić Department of Organic chemistry, Faculty of Technology, University of Tuzla, Bosnia and Herzegovina
  • Enida Karić Department of Pharmaceutical technology, University of Tuzla, Faculty of Pharmacy, Tuzla, Bosnia and Herzegovina

Keywords:

vaginal suppository, Melaleuca alternifolia, Achillea millefolium, Cinnamomum camphora, antimicrobial activity

Abstract

Vaginal inflammations represent a heterogeneous group of disorders caused by infection, inflammation or disruption of the vaginal microflora. The most common causes of vaginal infections are: Staphylococcus aureus, Enterococcus faecalis, Streptococcus agalactiae, Escherichia coli, Candida albicans. Antibiotic resistance is a major global problem, and this problem can be reduced by using natural antimicrobial substances such as essential oils. Each essential oil has an extremely complex composition (some essential oils have over 200 components), which prevents microorganisms from creating resistance - therefore, essential oils always retain their effect against them.  

The aim of our study was to investigate antibacterial activity Melaleuca alternifolia, Achillea millefolium and Cinnamomum camphora vaginal suppositories, and see which essential oil has the strongest potential to be used as active ingridient for vaginal infections.

Examination of the antimicrobial activity of vaginal suppositories was examined by the disk diffusion method. There were used standard bacterial strains from ATCC collection: Staphylococcus aureus (S. aureus) ATCC 25923, Enterococcus faecalis (E. faecalis) ATCC 51299, Escherichia coli (E. coli) ATCC 25922, Candida albicans (C. albicans) ATCC 10231.

The results showed that Melaleuca alternifolia essential oil has an antimicrobial effect on all tested strains, and the strongest on Candida albicans (ZI 22.7 mm). Achillea millefolium essential oil has no effect on Enterococcus faecalis, while Cinnamomum camphora essential oil did not show zones of inhibition on Candida albicans

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Published

2023-10-03

Issue

Vol. 16 No. 1 (2023)

Section

Articles

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Copyright (c) 2023 Merima Ibišević, Saša Pilipović, Darja Husejnagić, Fadila Malohodžić, Alma Kulanić, Lejla Mustafić, Ermina Cilović Kozarević, Emir Horozić, Enida Karić

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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