Original scientific paper
BACTERIOLOGICAL PROPERTIES OF MARINE WATER IN ADRIATIC FISH FARMS: ENUMERATION OF HETEROTROPHIC BACTERIA
Damir Kapetanović
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Aquaculture and Pathology of Aquatic Organisms, Bijenička cesta 54, 10000 Zagreb, Croatia;
Zrinka Dragun
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Biological Effects of Metals, Bijenička cesta 54, 10000 Zagreb, Croatia
Damir Valić
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Aquaculture and Pathology of Aquatic Organisms, Bijenička cesta 54, 10000 Zagreb, Croatia;
Irena Vardić Smrzlić
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Aquaculture and Pathology of Aquatic Organisms, Bijenička cesta 54, 10000 Zagreb, Croatia;
Zlatica Teskeredžić
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Aquaculture and Pathology of Aquatic Organisms, Bijenička cesta 54, 10000 Zagreb, Croatia;
Emin Teskeredžić
; Ruđer Bošković Institute, Division for Marine and Environmental Research, Laboratory for Aquaculture and Pathology of Aquatic Organisms, Bijenička cesta 54, 10000 Zagreb, Croatia;
Abstract
Aquaculture is currently one of the fastest growing food production sectors in the world. Increase in nutrients and organic wastes lead to general deterioration of water quality. The problem of water quality is associated with both physical and chemical factors, as well as microbiological water quality. Heterotrophic bacteria play an important role in the process of decomposition of organic matter in water environment and indicate eutrophication process.
Here we present our experience and knowledge on bacterial properties of marine water in the Adriatic fish farms with European sea bass (Dicentrarchus labrax L., 1758), with an emphasis on enumeration of heterotrophic bacteria in marine water.
We applied two temperatures of incubation, as well as two methods for enumeration of heterotrophic bacteria: substrate SimPlate® test and spread plate method on conventional artificial media (Marine agar and Tryptic Soy agar with added NaCl).
The results of analysis of bacteriological properties of marine water in the Adriatic fish farms showed that enumeration of heterotrophic bacteria in marine water depends on the applied incubation temperature and media for enumeration. At the same time, the incubation temperature of 22C favours more intense growth of marine heterotrophic bacteria, whereas a SimPlate test gives higher values of heterotrophic bacteria. Volatile values of heterotrophic bacteria during this research indicate a possible deterioration of microbiological water quality in the Adriatic fish farms and a need for regular monitoring of marine water quality.
Keywords
heterotrophic bacteria; European sea bass; SimPlate; marine agar; tryptic soy agar
Hrčak ID:
97744
URI
Publication date:
31.1.2012.
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