Izvorni znanstveni članak
In vitro germination of pollen grains of wild fig (Ficus carica L. var caprificus)
Dijana Vego
; Agronomski i prehrambeno-tehnološki fakultet Sveučilišta u Mostaru, Mostar, BIH
Ivo Miljković
; Čazmanska 2, Zagreb, Croatia
Sažetak
The aim of this study is to determine pollen germination and germination energy of selected types of wild fig in the region of Herzegovina. Pollen from wild fig was collected during anthers cracking from the end of June to the beginning of July. The pollen germination and germination energy for 12 types of wild fig were found. The pollen germination was researched by Lindler’s method of hanging cap. The germination energy was established on substrate with 1% agar-agar and 10% solution of sucrose in Petri dishes on the temperature of 21°C. The pollen fixation in Petri dishes was carried out with formaldehyde. The results in percents were obtained after counting germinated pollen grains under a microscope binocular and after analysing the situations on 5 observations for places germination and germination energy. It was established that the best conditions in researching pollen germination of wild fig were in 3% solution of sucrose and 0.01% solution of boric acid on temperature of 30 ºC and the best conditions in researching on germination energy were in 3% solution of sucrose + 1% agar-agar + 0.01% of boric acid on temperature of 30 ºC. The best pollen germination and germination energy was established for types 4 and 2.
Ključne riječi
types; caprification; pollen germination; germination energy
Hrčak ID:
102046
URI
Datum izdavanja:
10.5.2013.
Posjeta: 2.080 *