Original scientific paper
Genetic Diversity of Dalmatian Sage (Salvia officinalis L.) as Assessed by RAPD Markers
Zlatko Liber
; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany and Botanical Garden, Marulićev trg 9A, 10000 Zagreb, Croatia
Vesna Židovec
; University of Zagreb, Faculty of Agriculture, Department of Ornamental Plants, Landscape Architecture and History of Garden Art, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Sandro Bogdanović
; University of Zagreb, Faculty of Agriculture, Department of of Agricultural Botany, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Ivan Radosavljević
; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany and Botanical Garden, Marulićev trg 9A, 10000 Zagreb, Croatia
Monika Pruša
; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany and Botanical Garden, Marulićev trg 9A, 10000 Zagreb, Croatia
Maja Filipović
; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany and Botanical Garden, Marulićev trg 9A, 10000 Zagreb, Croatia
Ines Han Dovedan
; University of Zagreb, Faculty of Agriculture, Department of Ornamental Plants, Landscape Architecture and History of Garden Art, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Marija Jug-Dujaković
; Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia
Zlatko Šatović
; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Abstract
Dalmatian or common sage (Salvia officinalis L.) is an outcrossing plant species native to East Adriatic coast. Random Amplified Polymorphic DNA markers (RAPD) were used to analyze genetic diversity and structure of ten natural populations from the East-Adriatic coastal region. The highest genetic diversity was found in populations from the central and south Dalmatia, while the highest frequency down-weighted marker values were found in the northernmost populations and the southern most inland population. Although analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, highly significant φST values suggested the existence of genetic differentiation among populations.
By assuming Hardy-Weinberg equilibrium within populations, the calculated FST value among population was moderate. Bayesian model-based clustering method revealed that at K = 2 all individuals belonging to two northern populations were assigned to a separate cluster from the individuals belonging to the rest of the population. At K = 3, the newly formed cluster grouped the majority of individuals belonging to populations from central Dalmatia. The high correlation between matrices of genetic and geographical distances showed that isolation by distance may play a considerable role in overall structuring of the genetic diversity.
Keywords
Salvia; population genetics; RAPDs; genetic structure; isolation by distance
Hrčak ID:
129084
URI
Publication date:
18.6.2014.
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