Kratko priopćenje

https://doi.org/10.17113/ftb.53.03.15.3911

Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching

Karol Alfaro ; Centro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile
Paulina Bustos ; Centro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile
Ciara O´Sullivan ; Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili,Tarragona, Spain
Pablo Conejeros ; Centro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile

Sažetak

A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL.

Ključne riječi

red tide; paralytic shellfish poison; aptamers; saxitoxin; high resolution melting

Hrčak ID:

145510

URI

https://hrcak.srce.hr/145510

Datum izdavanja:

10.9.2015.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.586 *