Original scientific paper

Occurrence of 5-hydroxytryptamine in the Roman Nettle (Urtica pilulifera L.)

Ivan Regula ; Hrvatska

Abstract

Leaves and stems of the Roman nettle (Urtica pilulifera) were frozen, macerated in a blendor in 80% methanol at 5°C, and the plant material repeatedly extracted with methanol for 22 hrs at +2 °C. The separation of the basic compounds was achieved on a Amberlite CG-50 column. The identification of 5-hydroxytryptamine was based on Rf-values (compared to those of an authentic sample of 5-hydroxytryptamine), colour reactions with several reagents and fluorescence characteristics. The amount found was 0.4 p,g/g of fresh weight. Histochemical investigations with p-dimethylamino-cinnamaldehyde indicate that 5-hydroxytryptamine is located mainly in the stings.

Keywords

Hrčak ID:

156341

URI

https://hrcak.srce.hr/156341

Publication date:

31.12.1970.

Article data in other languages: croatian

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