Acta Botanica Croatica, Vol. 33 No. 1, 1974.
Izvorni znanstveni članak
Occurrence of Celery Mosaic Virus in Parsnip in Yugoslavia
Ivan Butorac
; Hrvatska
Ana Šarić
; Hrvatska
Nikola Ljubešić
; Hrvatska
Sažetak
A filamentous virus 740 nm in length and 13.5 nm in width was isolated from parsnip (Pastinaca sativa) showing mosaic symptoms. The virus was easily transmissible by sap and also by aphid Myzus persicae in a non persistent manner. It had a restricted host range, infecting systemically only members of the Umbelliferae (except Ammi majus). In the leaf cells of Anthriscus cerefolium it induced the formation of pinwheel structures.
According to its properties in vitro, its host range, particle morphology and the serological reactions obtained it was identified as a strain of celery mosaic virus related to but not identical with the isolates from England, Italy and East Germany.
The purification treatment by chloroform clarification followed by polyethyleneglycol precipitation and concentration by differential centrifugation produced preparations free of host material although containing somewhat fewer virus particles. For obtaining a good yield of virus it was necessary to use buffers containing substances which prevent the oxidation of sap and the aggregation of virus particles.
This is the first report of celery mosaic virus in Yugoslavia.
Ključne riječi
Hrčak ID:
157683
URI
Datum izdavanja:
31.12.1974.
Posjeta: 1.284 *