Izvorni znanstveni članak

Shoot and root regeneration from callus tissue of Allium commutatum Guss.

Marija Vujević ; University of Zagreb, Faculty of Science, Department of Botany, Zagreb, Croatia
Branka Pevalek-Kozlina ; University of Zagreb, Faculty of Science, Department of Botany, Zagreb, Croatia
Mirjana Pavlica ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia
Marija Edita Šolić ; Institute "Planina i More", Makarska, Croatia

Sažetak

Callus tissue was induced on root tips of in vitro cultured seedlings of Allium commutatum Guss. on MS medium supplemented with 3 % sucrose, 0.8 % agar, 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.6 μM kinetin. Pieces of developed calli were transferred to MS medium with different concentrations of 2,4-D (0.02, 0.05 and 0.1 μM) and kinetin (0.02, 0.1 and 0.5 μM) or without them. After five weeks of cultivation, callus proliferation and differentiation as well as adventitious shoot and root regeneration were analysed. The best callus proliferation and adventitious root induction were achieved on MS medium containing 0.1 and 0.5 μM kinetin; addition of 2,4-D had no significant influence. Higher concentrations of kinetin also favoured higher incidence of meristemoids. Adventitious shoot development was noticed only on three media tested.

Ključne riječi

Allium commutatum; callus culture; differentiation; regeneration

Hrčak ID:

160597

URI

https://hrcak.srce.hr/160597

Datum izdavanja:

31.12.1999.

Posjeta: 929 *