Original scientific paper

https://doi.org/10.24099/vet.arhiv.161229

Detection of Babesia canis vogeli, Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India

Jose Jain ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Bindu Lakshmanan orcid.org/0000-0002-5138-5620 ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Hitaishi V. Nagaraj ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Jose E. Praveena ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Karunakaran Syamala ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Thazhathuveetil Aravindakshan ; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India

Abstract

Tick borne haemoparasites and haemorickettsiales pose a major health risk to animals worldwide. The present study reports the development and validation of multiplex PCR to simultaneously detect the most prevalent tick borne pathogens infecting dogs in Kerala, South India. The assay targeting the small subunit ribosomal RNA genes of the organisms could amplify well demarcated amplicons of B. canis vogeli, B. gibsoni and E. canis. In the study population, which included both healthy dogs as well as those with clinical symptoms suggestive of the three infections under study, 46.6% animals were infected with one of the three pathogens, amongst which the occurrence of B. gibsoni was significantly the highest. Natural co-infections were also detected in nine dogs, which suggests the suitability of the assay to assist in the selection of pathogen specific treatment protocols.

Keywords

Multiplex PCR; Babesia canis vogeli; Babesia gibsoni; Ehrlichia canis

Hrčak ID:

197973

URI

https://hrcak.srce.hr/197973

Publication date:

2.4.2018.

Article data in other languages: croatian

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