Veterinarski arhiv, Vol. 88 No. 2, 2018.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.161229
Dokaz vrsta B. canis vogeli, B. gibsoni i E. canis višestrukim PCR-om u prirodno inficiranih pasa u Južnoj Indiji
Jose Jain
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Bindu Lakshmanan
orcid.org/0000-0002-5138-5620
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Hitaishi V. Nagaraj
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Jose E. Praveena
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Karunakaran Syamala
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Thazhathuveetil Aravindakshan
; School of Applied Animal Production and Biotechnology, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India
Sažetak
Krvni paraziti i rikecije podrijetlom iz krpelja glavna su opasnost za zdravlje životinja diljem svijeta. U ovom je istraživanju razvijen i validiran višestruki PCR za istodobno dokazivanje najčešćih uzročnika koje na pse prenose krpelji u Kerali u Južnoj Indiji. Malim ciljnim subjediničnim ribosomskim RNA mogli su proizvesti jasno razlučivi produkti specifični za B. canis vogeli, B. gibsoni i E. canis. U pretraživanoj populaciji koja je obuhvaćala zdrave pse i one s kliničkim znakovima, koji su upućivali na infekciju uzrokovanu jednim od spomenutih uzročnika, 46,6 % životinja bilo je inficirano jednim od njih triju. Infekcija vrstom B. gibsoni bila je znatno češća. Prirodna je koinfekcija dokazana u devet pasa, što potvrđuje prikladnost testa za dijagnostiku i njegovo značenje pri poduzimanju odgovarajućeg liječenja.
Ključne riječi
višestruki PCR; Babesia canis vogeli; Babesia gibsoni; Ehrlichia canis
Hrčak ID:
197973
URI
Datum izdavanja:
2.4.2018.
Posjeta: 2.402 *