Original scientific paper
Quantitative Trait Locus Analysis of Plant Height– Related Traits in Cucumber Using F2:3 Population
Hamid Kalvandi
orcid.org/ORCID
; University of Guilan, University Campus 2, Department of Horticultural Science, Rasht, Iran
Jamal-Ali Olfati
orcid.org/ORCID
; University of Guilan, University Campus 2, Department of Horticultural Science, Rasht, Iran; University of Guilan, Faculty of Agricultural Sciences, Department of Horticultural Sciences, Rasht, Iran
Habibollah Samizadeh
orcid.org/ORCID
; University of Guilan, Faculty of Agricultural Sciences, Department of Agronomy and Plant Breeding, Rasht, Iran
Yavar Vafaee
orcid.org/ORCID
; University of Kurdistan, Faculty of Agricultural Sciences, Department of Horticultural Sciences, Sanandaj, Iran
Abstract
In present study, C18 (determinate) and C19 (indeterminate) cucumber lines were crossed and the hybrid plants were self-pollinated to produce the F2:3 generation. Thirteen ISSR (Inter Simple Sequence Repeat) primers, two retrotransposons and two IRAP (Inter-Retrotransposon Amplified Polymorphism) combination primers were screened for QTLs (Quantitative Trait Loci) controlling plant height in cucumber. Five QTLs using SIM (Single Interval Mapping) were identified but only two major of them were observed using CIM (Composite Interval Mapping) method for plant height in F2:3 generation. The distance of one of the identified QTLs to nearest marker was very low and 0.01 CentiMurgan (cM), which showed a high linked marker to this QTL.
Keywords
ISSR; IRAP; ERAP (Exon-Retrotransposon Amplified Polymorphism); QTL; Retrotransposon
Hrčak ID:
203013
URI
Publication date:
10.6.2018.
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