Izvorni znanstveni članak

https://doi.org/10.24099/vet.arhiv.0192

Association of Aquaporin 7 gene variants with semen quality in Murrah buffaloes

Ragini Kumari ; Department of Animal Genetics and Breeding, Indian Council of Agricultural Research - National Dairy Research Institute, Karnal, India
Ramesha P. Kerekoppa ; Dairy Production Section, Indian Council of Agricultural Research-National Dairy Research Institute, Southern Regional Station, Bengaluru, India
Rakesh Kumar ; ICAR Research Complex for NEH Region, Umiam, Umroi Road, Meghalaya, India
Divya P ; Central Research Station, BAIF, Pune, India
Beena Sinha ; Department of Animal Genetics and Breeding, Indian Council of Agricultural Research - National Dairy Research Institute, Karnal, India
Marappa Basavaraju ; Dairy Production Section, Indian Council of Agricultural Research-National Dairy Research Institute, Southern Regional Station, Bengaluru, India
Akhila Rao ; Dairy Production Section, Indian Council of Agricultural Research-National Dairy Research Institute, Southern Regional Station, Bengaluru, India

Sažetak

The Aquaporin 7 (AQP7) gene, a member of the aqua-glyceroporins, was characterised using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) analysis, in order to investigate the association of genetic variants of the AQP7 gene with semen quality in Murrah bulls. In this study, genomic DNA was extracted from the blood of Murrah bulls (n = 69) using the high salt method. Sequence data were analyzed using Bio Edit software (version 7.0.5) for detecting single nucleotide polymorphisms (SNPs). Statistical analysis was carried out using repeated GLM procedure measures (SAS 9.3). Analysis revealed 17 different SSCP variants in the AQP7 gene in Murrah buffaloes. Three unique SSCP band patterns were observed in exons 1 and 4. Exons 5, 7 and 8 showed five, four and two unique SSCP band patterns, respectively. Sequence analysis revealed a total of 20 SNPs (8 in exonic and 12 in intronic region). PCR-SSCP variants of exon 5 were significantly associated (P<0.01) with sperm concentration. SSCP variants of exons 5 and 8 were associated (P<0.05) with sperm viability and post thaw motility (PTM), respectively. The study revealed a high degree of genetic variability in the AQP7 gene in Murrah bulls. The SSCP variants observed are associated with semen parameters which suggests the possibility of using the AQP7 gene as a candidate gene for identification of markers for semen quality traits in buffaloes.

Ključne riječi

AQP7 gene; Murrah buffalo; PCR-SSCP; SNP

Hrčak ID:

214756

URI

https://hrcak.srce.hr/214756

Datum izdavanja:

15.12.2018.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.089 *