Original scientific paper

Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Nándor Nemestóthy ; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary
László Gubicza ; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary
Erika Fehér ; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary
Katalin Bélafi-Bakó ; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary

Abstract

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.

Keywords

fusel oil; esterification; lipase; ping-pong bi-bi mechanism

Hrčak ID:

22214

URI

https://hrcak.srce.hr/22214

Publication date:

14.3.2008.

Article data in other languages: croatian

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