Original scientific paper
https://doi.org/10.5513/JCEA01/21.3.2705
Mitosis inhibitory and clastogenic effects of waters from anthropogenically affected areas
Ivan STOYANOV
orcid.org/0000-0002-5943-399X
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Penka VASILEVA
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Teodora STAYKOVA
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Veronika IVANOVA
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Teodora POPOVA
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Evgeniya IVANOVA
; Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, 24, Tzar Assen Str., Plovdiv, Bulgaria
Abstract
The present study aims to analyse the effect of waters, anthropogenically influenced by various pollutants, on the mitotic division and chromosomal apparatus of cells by establishing their potential mitoinhibitory and clastogenic effect. The cytotoxic and mutagenic effect of contaminated water was examined by the application of the Allium cepa test system. Mitotic depression has been established for samples with available anthropogenic contamination. Microscopic analysis showed an increased incidence of chromosomal aberrations in the test samples compared to the control, resulting from the genotoxic effect available. Chromosomal abnormalities of the type of lagging and ‘vagrant’ chromosomes, chromosomal fragments, anaphase and telophase bridges, micronuclei, as well as deviations from normal cell division
such as K-mitoses and asynchronous mitoses have been observed. The analysis of the spectrum of chromosomal aberrations shows some differences in the frequency of occurrence of the different types of disorders, which reflects the specificity of the genotoxic effect of the water samples from the surveyed areas.
Keywords
Allium test; chromosomal aberration; cytotoxicity; genotoxicity; mitotic index
Hrčak ID:
244316
URI
Publication date:
29.9.2020.
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