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Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White

Ivo Safarik orcid.org/0000-0002-3108-7319 ; Department of General Biology, Faculty of Biological Sciences, University of South Bohemia, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic
Zdenka Sabatkova ; Department of Biomagnetic Tehniques, Institute of Systems Biology and Ecology, Academy of Sciences, Na Sadkach 7, CZ-37005 Ceske Budejovice, Czech Republic
Oldrich Tokar ; Iontosorb, Anezky Ceske 657/33, CZ-40007 Usti nad Labem, Czech Republic
Mirka Safarikova ; Department of Biomagnetic Tehniques, Institute of Systems Biology and Ecology, Academy of Sciences, Na Sadkach 7, CZ-37005 Ceske Budejovice, Czech Republic

Sažetak

Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.

Ključne riječi

hen egg white; lysozyme; magnetic separation; cellulose cation exchangers

Hrčak ID:

23092

URI

https://hrcak.srce.hr/23092

Datum izdavanja:

13.12.2007.

Podaci na drugim jezicima: hrvatski

Posjeta: 5.547 *