Veterinarski arhiv, Vol. 94 No. 2, 2024.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.2129
The role of oxidative and nitrosative stress in bovine ocular squamous cell carcinomas
Emin Karakurt
; Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Uğur Aydın
; Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Enver Beytut
; Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Serpil Dağ
; Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Özgür Aksoy
; Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Hilmi Nuhoğlu
; Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Uğur Yıldız
; Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Ayfer Yıldız
; Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
Sažetak
Oxidative stress is strongly linked to carcinogenesis, especially head, neck and oral SCCs in humans. In this study, we aimed to evaluate the role of oxidative and nitrosative stress in Bovine ocular squamous cell carcinomas (BOSCCs) using immunohistochemical markers such as Nitrotyrosine (NT), Malondialdehyde (MDA) and 8-hydroxy-2’ -deoxyguanosine (8-OHdG). Tissues were collected from 24 cattle brought to the Pathology Department for routine diagnosis. Tumour samples were fixed in 10% neutral buffered formalin, processed routinely, and embedded in paraffin wax. Tissue sections were cut into 5 μm thickness and stained with Hematoxylin-Eosin to detect histopathological changes. The sections were examined under a light microscope and photographed. The avidin-biotin-peroxidase method was used as immunohistochemical staining. It was observed that the cauliflower-like tumoral masses were mostly located on the upper and lower eyelids, the third eyelid, cornea, limbus, or covering the entire eyeball. NT, MDA and 8-OHdG expressions were statistically increased in poorly-differentiated (PD) cases compared to welldifferentiated (WD) and moderately-differentiated (MD) cases. On the basis of the results of the increase in these markers, we concluded that nitrosative and oxidative stress may have an important role in the carcinogenesis of BOSCCs.
Ključne riječi
bovine; nitrosative stress; oxidative stress; squamous cell carcinoma
Hrčak ID:
316567
URI
Datum izdavanja:
18.3.2024.
Posjeta: 49 *