Original scientific paper
Influence of maceration, solvent types, and extraction durations on the yield of milk thistle seeds (Silybum Marianum) extraction
Halid Makić
; Biotechnical Faculty, University of Bihać, Bihać, Bosnia and Herzegovina
Samira Hotić
; Biotechnical Faculty, University of Bihać, Bihać, Bosnia and Herzegovina
Elvisa Hodžić
orcid.org/0000-0003-3421-2426
; Biotechnical Faculty, University of Bihać, Bihać, Bosnia and Herzegovina
Nenad Stojanović
; Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzegovina
Jasmina Ibrahimpašić
; Biotechnical Faculty, University of Bihać, Bihać, Bosnia and Herzegovina
Samira Dedić
; Biotechnical Faculty, University of Bihać, Bihać, Bosnia and Herzegovina
Emina Ćehajić Gradinović
; International Organization for Migration, Bihać, Bosnia and Herzegovina
Abstract
ABSTRACT:
The study investigated the extraction yield of defatted Silybum marianum seed samples using maceration as the sole extraction technique. Different solvent types (methanol, ethanol, and water) and extraction durations were tested. Prior to extraction, the samples were ground and defatted with n-hexane. For each combination of solvent type, and extraction duration, the extracted mass (g of extract/g of defatted sample) was determined. The impact of each parameter on the yield was analyzed, revealing significant effects. Results showed that water-based maceration for 4 hours yielded the highest average mass of dry extract, followed by shorter durations at 2 hours. Ethanol occasionally outperformed methanol, particularly at the 2-hour mark, but methanol consistently produced lower yields across longer extraction durations. These findings emphasize the need for careful optimization of solvent type and extraction duration to maximize extraction yield. Subsequent analysis using Tukey's HSD test revealed significant differences in dry extract mass among solvents. Water yielded the highest at 2 and 4 hours, ethanol at 4 hours, and methanol at 4 hours as well.
Keywords: Silybum marianum; maceration; solvent types; plant extraction, yield analysis
Keywords
Hrčak ID:
329781
URI
Publication date:
8.11.2024.
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