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Journal of Electrochemical Science and Engineering, Vol. 15 No. 5, 2025.

Original scientific paper

https://doi.org/10.5599/jese.2698

Porcine gelatine detection via electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel

Rafa Radithya Swara ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Nazwa Alya Zahra ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Dea Hasna Tsary ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Biyas Aurora Nania Nevada ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Elisabeth Nasya Dominika Setyawati Putri ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Salma Nur Zakiyyah ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Yohanes Susanto Ridwan ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Yeni Wahyuni Hartati ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia
Irkham Irkham ; Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia *

* Corresponding author.

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Abstract

Maintaining the safety and proper labelling of food products is vital for communities around the world. One of the primary concerns in food safety is the unintentional presence of porcine derivatives such as gelatine in food products. This issue could affect the labelling accuracy of food products, religious dietary observances, regulatory compliance, and public health. Electrochemical immunosensors address this issue by rapidly, portably, and especially with high sensitivity, detecting porcine gelatine. The electrode was modified with CeO2 nanoparticles (NPs) synthesized via a green method using orange peel extract, which enhanced the immobilization of the bioreceptor on the electrode surface. Orange peel extract contains secondary metabolites that can act as stabilizing agents and prevent agglomeration during the synthesis process. The synthesized CeO2 NPs show a cube morphology, producing an average particle size of 12 nm with 77.31 % crystallinity. The performance of green synthesized CeO2 NPs modified electrode are successful for detecting porcine gelatine in solution and commercialized gelatine product, resulting in a limit of detection of 3.53 ppm. These results show the potential of green-synthesized CeO2 NPs for an electrochemical immunosensor for detecting porcine products.

Keywords

Food ingredient; electrochemical sensing; screen printed electrode; nanoceria; plant extract

Hrčak ID:

335039

URI

https://hrcak.srce.hr/335039

Publication date:

31.7.2025.

Visits: 200 *