Izvorni znanstveni članak
https://doi.org/10.15644/asc59/3/1
Prevalence of Glutathione-S-Transferase T and M Deletion Polymorphisms in Apical Periodontitis: a Two-Center Observational Study
Mirsad Kadić
; Sarajevska škola nauke i tehnologije, Odsjek za stomatologiju, Bosna i Hercegovina; Sveučilište u Banjoj Luci, Medicinski fakultet, Bosna i Hercegovina
Nađa Nikolić
orcid.org/0000-0002-8513-9313
; Sveučilište u Beogradu, Stomatološki fakultet, Implantološko-istraživački centar, Istraživački laboratoriji, Srbija
Jelena Carkić
; Sveučilište u Beogradu, Stomatološki fakultet, Implantološko-istraživački centar, Istraživački laboratoriji, Srbija
Anesa Kadić-Pirović
; Sveučilište u Banjoj Luci, Medicinski fakultet, Bosna i Hercegovina
Katarina Beljić-Ivanović
; Sveučilište u Beogradu, Stomatološki fakultet, Klinika za bolesti zuba, Srbija
Miroslav Andrić
orcid.org/0000-0003-4136-7901
; Sveučilište u Beogradu, Stomatološki fakultet, Zavod za oralnu kirurgiju, Srbija
Naida Hadžiabdić
; Sveučilište u Sarajevu, Stomatološki fakultet sa stomatološkim kliničkim centrom, Klinika za oralnu kirurgiju, Bosna i Hercegovina
Igor Đukić
; Sveučilište u Banjoj Luci, Medicinski fakultet, Klinika za oralnu hirurgiju, Bosna i Hercegovina
Aleksandar Jakovljević
orcid.org/0000-0001-6512-4934
; Sveučilište u Beogradu, Stomatološki fakultet, Odsjek za patološku fiziologiju, Srbija
Sažetak
Objectives: The primary objective of this study was to examine the potential association between glutathione S-transferases (GSTT1/GSTM1) deletion polymorphisms and the development of apical periodontitis (AP) in a population of patients at two university centers: the Faculty of Medicine at the University of Banja Luka in Bosnia and Herzegovina and the School of Dental Medicine at the University of Belgrade in Serbia. Materials and Methods: The study involved 200 patients with AP in the experimental and 250 healthy individuals without AP in the control group. As a source of genomic DNA, sterile buccal swabs were taken from each patient. Genotyping of GSTM1 and GSTT1 deletion polymorphisms was conducted using multiplex Polymerase Chain Reaction (PCR). The risk of AP development with regard to the genotypes was evaluated based on odds ratios (ORs) and 95% confidence intervals (CIs) that were calculated via unconditional logistic regression. Results: There were significant differences in demographic characteristics between the investigated groups (p = 0.446, p = 0.154, respectively). GSTM1 and GSTT1 deletions were associated with a 3.05-fold and 5.69-fold risk (OR = 3.05, 95% CI = 2.07–4.49, OR = 5.69, 95% CI = 3.66–8.86, p < 0.001, p < 0.001, respectively) for the AP development. The co-occurrence of both deletions posed a significantly higher risk for AP development (OR = 52.76. 95% CI = 18.20–152.94, P < 0.001). Conclusions: The carriers of null GSTT, null GSTM, and double null GSTT/GSTM genotypes are more susceptible to AP development in the populations examined at the two centers.
Ključne riječi
Periapical Periodontitis; Genetic Polymorphism; Glutathione S-Transferase; Apical periodontitis, Heredity, Oxidative stress, Deletion polymorphisms, Gluthathione-STransferase
Hrčak ID:
335350
URI
Datum izdavanja:
15.9.2025.
Posjeta: 233 *