Original scientific paper
https://doi.org/10.5599/jese.2807
Development of non-invasive genosensor to diagnose pancreatic cancer using miRNA-196a
Namita Sharma
orcid.org/0009-0009-4026-9593
; Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, India
Sudha Srivastava
; Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, India
*
* Corresponding author.
Abstract
This work presents the development of a diagnostic device for pancreatic cancer, one of the deadliest malignancies worldwide. The circulating pancreatic cancer biomarker miRNA-196a was utilized for the development of an electrochemical genosensor due to its stability in biological fluids. Immobilized single-stranded DNA conjugated with gold nanoparticles establishes a sensitive platform for the electrochemical detection of miRNA-196a. The sensor performance was evaluated for probe DNA-miRNA hybridization at optimum temperature (55 °C) using cyclic voltammetry and chronocoulometric techniques, achieving sensitivities of 51.10 µA pg-1 mL cm-2 and 18.28 µC pg-1 mL cm-2, respectively. The device exhibited a wide linear detection range of miRNA-196a from 14.9 aM to 14.9 nM. To the best of our knowledge, for already developed PDAC biosensors using a single biomarker or a panel of biomarkers, the widest detection range for miRNA-196a was reported as 0.05 fM to 50 pM by Guo et al. in 2018. Hence, the developed genosensor, having a detection limit of 14.9 aM and a wide detection range, may prove to be a promising tool in detecting pancreatic cancer at an early stage, and hence improve patient outcomes.
Keywords
DNA biosensor; gold nanoparticles; hybridization; cyclic voltammetry; chronocoulometry; limit of detection
Hrčak ID:
339946
URI
Publication date:
15.9.2025.
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