Kratko priopćenje
https://doi.org/10.5599/jese.2867
Electrochemical assessment of α-amylase inhibition by type 2 diabetes drugs: a cyclic voltammetry study
Touhami Lanez
orcid.org/0000-0002-3978-7635
; University of El Oued, Faculty of Sciences, Chemistry Department, VTRS Laboratory, El Oued, Algeria
*
Elhafnaoui Lanez
; University of El Oued, Faculty of Nature and Life Sciences, Cellular and Molecular Biology, Department, VTRS Laboratory, El Oued, Algeria
Aicha Adaika
; University of El Oued, Faculty of Nature and Life Sciences, Cellular and Molecular Biology, Department, VTRS Laboratory, El Oued, Algeria
* Dopisni autor.
Sažetak
A new sensitive cyclic voltammetry method was developed for measuring the α-amylase inhibitory activity of six antidiabetic drugs commonly prescribed for type 2 diabetes: acarbose, amaryl, repaglinide, diamecron, dapagliflozin, and metformin. The electrochemical response of an enzyme-starch mixture was measured in the absence and presence of these drugs. Results indicated varying inhibitory potencies with IC50 values ranging from 6.770 to 41.423 µM, with acarbose as the most potent. UV-Vis absorption spectroscopy at 580 nm validated the cyclic voltammetry results, with very good concordance. This study highlights the utility of electrochemical assays for the rapid, quantitative screening of α-amylase inhibitors and supports their integration with spectroscopic methods for the evaluation of antidiabetic drugs.
Ključne riječi
Enzyme-starch mixture; IC50; electrochemical assay; antidiabetic agents
Hrčak ID:
344861
URI
Datum izdavanja:
2.11.2025.
Posjeta: 239 *