Izvorni znanstveni članak

Anti-oxidative/anti-inflammatory paraoxonase activity and lipid alterations after total splenectomy and autologous spleen transplantation in pigs.

Romana Turk ; Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Dražen Vnuk ; Clinic for Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine,
Ante Svetina ; Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Zlata Flegar-Meštrić ; Institute of Clinical Chemistry, Clinical Hospital “Merkur”, Zagreb, Croatia
Nika Brkljača Bottegaro ; Clinic for Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine,
Dubravka Juretić ; Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Sažetak

The objective of this study was to investigate serum PON1 activity and lipid and lipoprotein status after total splenectomy and autologous spleen transplantation in pigs, in order to assess the effect of surgical stress and trauma on PON1 activity and lipid metabolism. Nineteen piglets used in the experiment were randomly divided into three groups: sham-operation with spleens intact (n = 6), total splenectomy (n = 6), and splenic autotransplantation (n = 7) with small fragments of the spleen autotransplanted into the greater omentum. The blood samples were taken just before surgery and on the 1st, 5th, 12th, 26th and 40th days postoperatively. PON1 activity, total cholesterol and HDL-C were assayed in the sera. PON1 activity was significantly decreased postoperatively in splenectomized pigs (on days 5, 12 and 40) and in autotransplantated pigs (on days 5, 12, 26 and 40) while there were no PON1 changes in the sham-operated. In sham-operated pigs, total cholesterol was significantly decreased only on the 12th day postoperatively, while HDL-C was significantly decreased postoperatively on the 1st, 5th, 12th, 26th and 40th days with the minimum value on the 12th day. In splenectomized pigs total cholesterol was significantly decreased on the 5th, 12th, 26th and 40th days postoperatively, while HDL-C was decreased as well on the 1st, 5th, 12th and 40th days and both were the lowest on the 12th day. In pigs with autologous splenic transplants, total cholesterol was significantly decreased on the 12th, 26th and 40th days, while there was no difference in HDL-C. The results demonstrated decreased PON1 activity and alterations in the concentrations of total cholesterol and HDL-C after surgery due to surgical trauma and post-surgical inflammatory response.

Ključne riječi

paraoxonase; serum lipids; splenectomy; spleen autotransplantation; surgical trauma; inflammation

Hrčak ID:

32928

URI

https://hrcak.srce.hr/32928

Datum izdavanja:

24.2.2009.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.712 *