Acta Botanica Croatica, Vol. 63 No. 1, 2004.
Original scientific paper
Thidiazuron induced somatic embryogenesis in Coffea arabica L. and Coffea canephora P ex Fr.
P. Giridhar
V. Kumar
E.P. Indu
A. Chandrasekar
G.A. Ravishankar
Abstract
The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions of explants on TDZ (9.08 M)
containing medium within a period of two months. Subculturing of these embryo clusters produced more secondary embryos on reduced TDZ (0.045–0.91M) containing medium and these subsequently developed into plantlets (80–85%) on development medium followed by rooting on MS basal medium. This direct somatic embryogenesis from leaf and hypocotyl explants in Coffea sp. is a strong evidence of cell totipotency. The rapid somatic embryo induction protocol would be useful for the mass propagation, direct regeneration and genetic transformation of selected elite lines.
Keywords
Coffea arabica; Coffea canephora; cotyledons; hypocotyls; leaf; somatic embryos; thidiazuron
Hrčak ID:
3335
URI
Publication date:
15.4.2004.
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