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BLOOD ENZYMES AND THYROID HORMONES ACTIVITY FOR ASSESSMENT OF SELENIUM SUPPLY IN SHEEP
Zvonko Antunović
; Poljoprivredni fakultet Sveučilišta u Osijeku, Osijek, Hrvatska
Ivica Marić
; Hrvatski stočarski centar, Dubrovnik, Hrvatska
Ivica Matanić
; Alltech Biotehnologija d.o.o. Zagreb, Hrvatska
Đuro Senčić
; Poljoprivredni fakultet Sveučilišta u Osijeku, Osijek, Hrvatska
Marcela Šperanda
; Poljoprivredni fakultet Sveučilišta u Osijeku, Osijek, Hrvatska
Josip Novoselec
; Poljoprivredni fakultet Sveučilišta u Osijeku, Osijek, Hrvatska
Sažetak
The aim of this investigation is to determine whether the activity of enzymes and thyroid hormones in the blood of sheep and lamb can be used as a reliable criterion in assessing their supply with selenium. The research was conducted on 30 nongravid sheep and 30 lambs Dubrovnik sheep, during the summer season of nutrition. The control group of sheep consumed pasture, hay and forage mixture (200 g/day), while the control group of lambs consumed the mixture ad libitum. In the experimental group of sheep and lambs in a mixture of fodder inorganic (sodium selenite) or organic source (Sel-Plex) of selenium was added in the amount of 0.03% dry matter. It is not found significant deviation in the activities of most enzymes in the blood of sheep and lamb, depending on the group, but it is visible trend of increasing activity of enzymes in the control group compared to the experimental group. Statistically significant ((P<001) higher activity of GSH-PX in blood was found in sheep that consumed fodder mixture with the addition of organic and inorganic selenium compared to the control group and the lambs, which had consumed the mixture with the addition of organic selenium in relation to the other two groups. Statistically significant (P< 0.05) lower activity of T4 was determined in the blood of sheep that consumed added selenium in relation to the control group of sheep. These changes in GSH-PX activity suggest better use of organic in comparison to the inorganic form of selenium. Significantly higher (P<0.05) activity of ALT and LDH (on upper limit physiological values) was found in the blood of the control group lamb in relation to the group that had consumed the mixture with the addition of organic selenium. Mentioned changes activity of enzymes and thyroid hormones in the blood of sheep and lambs showed the justification of adding selenium, especially organic selenium in food lambs, with regard to increased need for selenium in many metabolic processes that follow their intensive growth. The activity of enzymes and thyroid hormones in the blood of sheep and lambs can be taken as a reliable criterion in assessing their supply with selenium.
Ključne riječi
sheep; lambs; selenium; enzymes; thyroid hormones; blood
Hrčak ID:
50647
URI
Datum izdavanja:
25.9.2009.
Posjeta: 1.447 *