Original scientific paper

Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application

Enida Članjak ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Muhamed Smajlović ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Faruk Čaklovica ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Davor Alagić ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Kenan Čaklovica ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Ahmed Smajlović ; Department of Pharmacology and Toxicology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina

Abstract

The paper presents detection results of enrofloxacin residues in muscle tissue and liver tissue of broiler chickens after the experimental application of prophylactic and therapeutic doses of the investigated drug. Two methods were used for the detection of enrofloxacin residues in muscle and liver tissue, and they are: microbiological method (growth inhibition test) and ELISA test. The aim of this research was to examine the reliability of the microbiological growth inhibition test (diffusion method) with the application of reference strain E. coli ATCC 10 536 as the microorganism- test for the detection of enrofloxacin residues in broiler meat and to compare the applied methods. By using phi correlation coefficient it was determined that there is a statistically very significant positive correlation (p <<0.001) between the data of the microbiological and ELISA method and in samples of muscle tissue and liver tissue. There was also determined that, in experimental conditions, both microbiological and ELISA method achieve equally positive results in the detection
of the allowed quantities of enrofloxacin residues, although they are different measures (mm or ppb) of the same phenomenon.

Keywords

enrofloxacin; growth inhibition test; ELISA

Hrčak ID:

72580

URI

https://hrcak.srce.hr/72580

Publication date:

15.6.2011.

Article data in other languages: croatian

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