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Original scientific paper

https://doi.org/10.2478/v10184-011-0055-4

The correct phylogenetic position of Lotus conimbricensis Brot. (Leguminosae, Loteae) based on nuclear ribosomal ITS sequences

Miguel A. Faria ; REQUIMTE, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia da Universidade do Porto, Rua de Aníbal Cunha, 164, 4099-030 Porto, Portugal
D. James Harris ; CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal; Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 4099-002 Porto, Portugal
Tatiana Visnevchi - Necrasov ; CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
Manuel Tavares De Sousa ; Estação Nacional de Melhoramento de Plantas, Estrada de Gil Vaz, 7350-951 Elvas, Portugal
Eugenia Nunes ; CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal


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Abstract

The nrDNA ITS sequence determined in Lotus conimbricensis in a previous phylogenetic study was unusual, in that it was almost identical to those retrieved from the morphologically distinct species L. subbiflorus. In the present study we sequenced new specimens of both species to reassess the phylogenetic position of L. conimbricensis. We conclude that the ITS sequence of L. conimbricensis used in the earlier analyses was most likely erroneous, and in fact L. conimbricensis is not closely related to L. subbiflorus. Critical reexamination of previously published data indicates that several other similar errors may exist for other Lotus species, and these should be checked before taxonomic conclusions are made.

Keywords

Lotus conimbricensis; Lotus subbiflorus; ITS; phylogeny; taxonomy; nrDNA

Hrčak ID:

79029

URI

https://hrcak.srce.hr/79029

Publication date:

27.3.2012.

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