Preliminary communication

Enhanced Production of Thermostable Pullulanase Type 1 Using Bacillus cereus FDTA 13 and Its Mutant

Subhash U. Nair ; Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India
Rekha S. Singhal ; Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India
Madhusudan Y. Kamat ; Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India

Abstract

This paper describes optimization of fermentation conditions for the production of thermostable pullulanase from a new strain Bacillus cereus FDTA 13, isolated from tapioca soil. By using one-factor-at-a-time and orthogonal array method, a simple medium with effective and minimal components was optimized for maximal pullulanase production. The optimized medium containing (in g/L): starch 20, yeast extract 5, NaCl 2, MgSO4 0.1 and K2HPO4 0.17 gave nearly 3.6-fold increase in pullulanase activity. Addition of Mn2+ ions in the optimized medium further enhanced the production by 1.4-fold. Effects of proteolytic inhibitors on stability of pullulanase in the medium were studied. A mutant Bacillus cereus FDTA 13/NTG04-B4, obtained by chemical mutation of the parent strain, further increased the pullulanase production by 2-fold in optimized medium, and gave similar yield as the parent strain in a medium with lower levels of carbon source.

Keywords

thermostable pullulanase; Bacillus cereus; orthogonal array; optimization; mutation; polymethylsulphonate fluoride

Hrčak ID:

109899

URI

https://hrcak.srce.hr/109899

Publication date:

15.6.2006.

Article data in other languages: croatian

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