Original scientific paper
Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method
Marcin Skowronek
; Department of Industrial Microbiology, Maria Curie-Sk³odowska University, Akademicka 19, PL-20-033 Lublin, Poland
Jan Fiedurek
; Department of Industrial Microbiology, Maria Curie-Sk³odowska University, Akademicka 19, PL-20-033 Lublin, Poland
Abstract
The simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method. The greatest advantage of the simplex method is that the optimum factor levels were determined in only 12 iterative steps, shortening a typical optimum search process considerably. This is the first use of the simplex method for optimising inulinase production.
Keywords
simplex optimisation; inulinase; Aspergillus niger
Hrčak ID:
110824
URI
Publication date:
15.9.2004.
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