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ADMET and DMPK, Vol. 2 No. 4, 2014.

Original scientific paper

https://doi.org/10.5599/admet.2.4.42

Potential of imaging analysis in establishing skin concentration-distance profiles for topically applied FITC-dextran 4 kDa

Shosho Kijima ; Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama 350 - 0295, Japan
Ryosuke Masaki ; Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama 350 - 0295, Japan
Wesam R. Kadhum ; Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama 350 - 0295, Japan
Hiroaki Todo ; Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama 350 - 0295, Japan
Tomomi Hatanaka ; Institute of Innovative Science and Technology, Medical Science Division, Tokai University, 4 - 1 - 1 Kitakaname, Hiratsuka, Kanagawa 259 - 1292, Japan
Kenji Sugibayashi ; Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama 350 - 0295, Japan

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Abstract

Quantitatively determining the skin concentration-distance profiles of topically applied drugs is important for evaluating their safety and efficacy. The aim of the present study was to quantitatively visualize the distribution of hydrophilic drugs through the skin using confocal laser scanning microscopy (CLSM) in order to obtain skin concentration-distance profiles. FITC-dextran with a molecular weight of approximately 4 kDa (FD-4) was selected as the model fluorescent drug in the present study, and excised pig ear skin was used. The skin concentration of FD-4 at each depth of a skin section was assessed by imaging analysis of the intensity of fluorescence. The FD-4 skin concentration-distance profile obtained was analyzed using Fick’s second law of diffusion, and was markedly similar to that using skin permeation parameters in the skin permeation study. These results suggest that the present CLSM method may be a promising tool for quantitatively visualizing the concentration-distance profiles of drugs through the skin.

Keywords

Confocal microscopy; FITC-dextran; dermatopharmacokinetics; skin permeation

Hrčak ID:

132855

URI

https://hrcak.srce.hr/132855

Publication date:

9.1.2015.

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