ADMET and DMPK, Vol. 3 No. 3, 2015.
Original scientific paper
https://doi.org/10.5599/admet.3.3.191
Prion protein and its interactions with metal ions (Cu2+, Zn2+, and Cd2+) and metallothionein 3
Branislav Ruttkay - Nedecky
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Eliska Sedlackova
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Dagmar Chudobova
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Kristyna Cihalova
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Ana Maria Jimenez Jimenez
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Sona Krizkova
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Lukas Richtera
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Vojtech Adam
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Rene Kizek
; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ - 613 00 Brno, Czech Republic, European Union
Abstract
The effects of heavy metals (Zn2+, Cu2+, and/or Cd2+) on Escherichia coli expressing either prion (hPrPC ) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrPC or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. After analyzing growth curves, we found that bacteria expressing prion protein better tolerated the presence of Zn2+ in comparison with wild-type bacteria and bacteria expressing MT-3. The addition of Cd2+ and Cu2+ was well tolerated by bacteria expressing MT-3, whereas the bacteria expressing prion protein displayed slower growth when compared to the wild-type. We subsequently determined total content of the MT in bacteria using differential pulsed voltammetry (DPV), and depending on the treatment of the individual metals. MT expression in MT3 transformed cells as well as in control E.coli cells increased at the lowest metal concentration (25 µM), followed by a decrease at higher metal concentrations (50, 75, and 150 µM). The highest increase by Cd2+ were observed. MT expression pattern in hPrPC transformed cells was different. After application of Cu2+ an increase in MT expression continued also at higher metal concentrations; and after application of Cd2+ and Zn2+ no decrease in MT expression at higher metal concentrations was observed.
Keywords
prion diseases; PrPC; PrPSc; MT; MT-3; copper; zinc; cadmium
Hrčak ID:
144321
URI
Publication date:
5.9.2015.
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