Acta Botanica Croatica, Vol. 48 No. 1, 1989.
Original scientific paper
The Allium-test Response to Cyanazine
Dražena Papeš
; Hrvatska
Višnja Besendorfer
; Hrvatska
Vesna Bosiljevac
; Hrvatska
Abstract
The effects of cyanazine on mitosis in Allium ce pa L. can be grouped as mitotic and chromosomal effects. Mitotic effects induced by delay in spindle formation were produced immediately after 3 hours treatments in all the concentrations.
The mitodepressive activities persisted in two highest concentrations (10-2 M and 10-3 M) only, whereas in solution of cyanazine intermediate concentrations (10-4 — 10-8 M) mitotic activity increased even more than in the control.
Chromosomal effects such as laggards, bridges, fragments, fragmentations and micronuclei were noticed after a 3-hours treatment but they were more intensively after 24- or 120-hours treatments in all the concentrations. Stickiness was very frequent and accompanied all the instabilities.
Comparison of these two effects shows that mitotic activity was parallel to the frequency of total changes in higher and intermediate concentrations.
Recovery from the cyanazine effects, after one day in water, caused normalization of mitotic activity, but a higher frequency of chromosome aberration continued in all the treated materials, depending on the concentration. We can conclude that there i no reversibility of cyanazine effects.
Keywords
Hrčak ID:
159510
URI
Publication date:
31.12.1989.
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