Acta Botanica Croatica, Vol. 49 No. 1, 1990.
Original scientific paper
Determination of Serological Differentiation Index Values of Turnip Yellow Mosaic Virus (TYMV) Strains by Means of Rocket Immunoelectrophoresis
Mladen Krajačić
; Hrvatska
Đorđe Mamula
; Hrvatska
Abstract
An attempt was made to apply rocket immunoelectro- phoresis (RIE) technique for the determination of serological differentiation index (SDI) values between three British strains of turnip yellow mosaic virus (TYMV) based on the height of »rocket« precipitates. The experiments were conducted in l°/o agar gel containing antiserum to Edinburgh (E) strain of the virus, the holes being charged separately with purified E and two other strains. Following experiments with E strain in several subsequent twofold dilutions, a calibration graph showing linear proportionality was drawn in a coordinate system determined by steps of virus dilutions and logarithm of »rockets« heights. By interpolation procedure SDIs of two heterologous strains against E were found fairly comparable with SDI values obtained by double radial immunodiffusion technique.
Keywords
Hrčak ID:
159554
URI
Publication date:
31.12.1990.
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