Original scientific paper

Ultrastructure and Protein Pattern of Horse-radish (Armoracia lapathifolia Gilib.) Crown-gall Tissue

Jasmina Muraja ; Hrvatska
Tatjana Peškan ; Hrvatska
Zdravko Lorković ; Hrvatska
Marijana Krsnik-Rasol ; Hrvatska

Abstract

Two horse-radish (Armoracia lapathifolia Gilib.) crown-gall tissue lines, one unorganized and the other teratoma producing were compared with the control leaf in ultrastructures and electrophoretic protein patterns. Tumour cells were similar to unspecialized parenchyma cells. During tumourigenesis the chloroplasts transformed into amyloplasts or leuko- plasts. Amylochloroplasts were present in teratoma cells. Large lobed nuclei were observed in both types of transformed cells. Leaf specific polypeptides of about 22-, 21-, 20- and 18 kDa disappeared in tumour extracts and the enzyme Rubisco quantitatively decreased. The polypeptide of about 85 kDa was present only in transformed tissues. In some other proteins only quantitative differences could be detected.

Keywords

Hrčak ID:

159962

URI

https://hrcak.srce.hr/159962

Publication date:

31.12.1994.

Article data in other languages: croatian

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