Original scientific paper

Jasmonic acid induces changes in growth and polypeptide composition of fern gametophytes

Marjana Camloh ; National Institute of Biology, Ljubljana, Slovenia
Barbara Vilhar ; Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia
Jana Žel ; National Institute of Biology, Ljubljana, Slovenia

Abstract

We studied morphology of gametophytes of the fern Platycerium bifurcation 20 to 45 days after spore sowing. In addition, we examined the effects of 0.01, 0.1, 1, 10 or 100 μM jasmonic acid (JA) on their growth and polypeptide composition. Gametophytes cultured for 20 days on modified Knop’s medium were oblong to round in outline. 3-5 rhizoids appeared mainly on the basal cell and 2-3 unicellular hairs formed on the margins. After 30 days in culture, slightly heart-shaped gametophytes produced numerous rhizoids and hairs. Gametophytes cultured for 45 days were cordate and the first antheridia were observed on the ventral side. Hairs, frequently branched, appeared on gametophyte surface. The effect of JA on the growth of gametophytes was age-dependent. After 20 and 30 days in culture, JA had no pronounced effect in comparison to the control (medium without JA), while after 40 days at concentrations exceeding 0.1 μM JA growth of gametophytes was inhibited. Analysis of soluble proteins from 2 month old JA-treated gametophytes revealed some alterations in polypeptide patterns when compared to the control. The most marked was an increase in a 92-93 kDa polypeptide band at 10 and 100 μM JA in the medium.

Keywords

Platycerium bifurcation; gametophyte; jasmonic acid; polypeptide; morphology; growth

Hrčak ID:

160831

URI

https://hrcak.srce.hr/160831

Publication date:

31.12.2001.

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