Original scientific paper
https://doi.org/10.17113/ftb.56.03.18.5348
Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28
Jelena Jović
orcid.org/0000-0001-9020-7854
; University of Belgrade, Faculty of Technology and Metallurgy, Department for Biochemical Engineering and Biotechnology, Karnegijeva 4, RS-11120 Belgrade, Serbia
Aneta Buntić
orcid.org/0000-0002-7068-1804
; University of Belgrade, Faculty of Technology and Metallurgy, Department for Biochemical Engineering and Biotechnology, Karnegijeva 4, RS-11120 Belgrade, Serbia
Neda Radovanović
orcid.org/0000-0002-2467-0629
; University of Belgrade, Faculty of Technology and Metallurgy, Department for Biochemical Engineering and Biotechnology, Karnegijeva 4, RS-11120 Belgrade, Serbia
Bojan Petrović
orcid.org/0000-0002-7182-917X
; University of Belgrade, Faculty of Technology and Metallurgy, Department for Biochemical Engineering and Biotechnology, Karnegijeva 4, RS-11120 Belgrade, Serbia
Ljiljana Mojović
orcid.org/0000-0001-7529-4670
; University of Belgrade, Faculty of Technology and Metallurgy, Department for Biochemical Engineering and Biotechnology, Karnegijeva 4, RS-11120 Belgrade, Serbia
Abstract
The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T. hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84 % with T. hirsuta F13 and 28.8 % with S. gausapatum F28.
Keywords
Trametes hirsuta; laccase; manganese peroxidase; Stereum gausapatum; lignin removal; white-rot fungi
Hrčak ID:
207634
URI
Publication date:
30.9.2018.
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