Original scientific paper

https://doi.org/10.20471/acc.2020.59.04.07

The Influence of Periodontal Disease Treatment on 8-Hydroxy-Deoxyguanosine Concentrations in Saliva and Plasma of Chronic Periodontitis Patients

Tanja Veljović orcid.org/0000-0002-9886-5488 ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Milanko Đurić ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; Department of Dentistry, Novi Sad, Serbia
Ivana Gušić ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; Department of Dentistry, Novi Sad, Serbia
Jelena Mirnić orcid.org/0000-0001-9637-8419 ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Saša Čakić ; University of Belgrade, Faculty of Dental Medicine, Department of Periodontology and Oral Medicine, Belgrade, Serbia
Aleksandra Maletin orcid.org/0000-0002-3655-6436 ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Snežana Brkić ; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; Department of Infectious Diseases, Clinical Centre of Vojvodina, Novi Sad, Serbia

Abstract

The 8-hydroxy-deoxyguanosine (8-OHdG) is one of the customary products of
oxidized DNA. The purpose of this study was to compare salivary and plasma 8-OHdG concentrations
in a group of chronic periodontitis patients to those measured in a group of patients with healthy
periodontium, as well as to determine the impact of periodontal therapy on 8-OHdG concentrations
in saliva and plasma in chronic periodontitis patients. The study sample comprised of 24 patients with
chronic periodontitis and 16 periodontally healthy individuals. Plaque index, gingival index, papilla
bleeding index, probing depth and clinical attachment level were indices used to determine patient
periodontal status. Salivary and plasma 8-OHdG concentrations were determined by ELISA method.
The salivary 8-OHdG concentration was statistically significantly higher in the group of periodontitis
patients compared to periodontally healthy subjects. After initial periodontal therapy, the 8-OHdG
concentration in saliva was significantly reduced in the periodontitis group (p=0.021). Differences in
plasma 8-OHdG concentrations between the two groups did not reach statistical significance and no
significant changes were noted in the periodontitis group following initial periodontal therapy. A
higher salivary 8-OHdG concentration reflects increased oxidative stress caused by periodontal disease.
Initial periodontal therapy may be helpful in reducing salivary 8-OHdG concentrations in
chronic periodontitis patients.

Keywords

Deoxyguanosine; Oxidative stress; Periodontal diseases; Saliva; Plasma

Hrčak ID:

253738

URI

https://hrcak.srce.hr/253738

Publication date:

1.12.2020.

Article data in other languages: croatian

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